Facts about semen liquefaction difficulties

Fresh ejaculated human semen is distinctly coagulated and the coagulum does not liquefy for at least 20 to 30 minutes. Commonly, semen samples are rather viscous even after 30-45 minutes, but beyond this, if liquefaction is not complete and the semen remains viscous, the indications are that some abnormality of the normal semen producing mechanisms exists and more specifically lack of an enzymatic process catalyzed by a proteolytic enzyme present in the prostatic secretions.

With the development of the various forms of assisted reproductive techniques (ART) and the need to extract the maximum numbers and quality of spermatozoa from the ejaculated semen has brought the semen viscosity into focus again. Viscous specimens are extremely difficult to manipulate in-vitro and may not allow the proper separation and isolation either the appropriate numbers, quality of sperm or both, necessary to achieve the in-vitro results. It is because of these reasons that the SpermPrep™ Viscolytic System (VLS) is introduced in the market to enable the proper in-vitro manipulation of viscous semen. The VLS can assist you prior to the performance of routine semen analysis and also the proper extraction of spermatozoa prior to their use in the various forms of ART including intra-uterine insemination.

Semen is collected by masturbation or other method such as using a special condom at intercourse. Sample should liquefy within 15-30 minutes of collection. If not, then employ the SpermPrep™ VLS1 Add 2.0 ml of SpermPrep™ medium to VLS. Add the VLS to the semen, mix gently and allow the mixture to set for 30 minutes. Wash the treated semen twice and process the sperm for IUI or other forms of ART.

If the reconstituted sample is to be filtered then follow protocol for use of SpermPrep™ II3.

PACKING SIZE 

SpermPrep™ VLS1 8 pcs/pack